摘要
采用聚丙烯酰胺垂直板状连续电泳的方法对青鱼（Mylopharyngodonpiceus）的脑、晶体、心脏、肾脏、肝脏、肌肉等六种组织进行了十种同工酶（α－AMY、EST、GOT、C6PDH、TDH、LDHMDH、ME、POX、SOD）的电泳研究，并对各种酶的同工酶位点表达及酶谱表型进行了分析。结果表明α－AMY、EST、TDH、LDH、MDH、ME、POX、SOD、C6PDH存在不同程度的组织特异性，COT无明显组织差异．并对同工酶的组织分布特异性的生理意义进行了讨论。
By using the vertical polyacrylamide gel electrophoresis, ten isozymes (α-AMY,EST GOT G6PDR IDH LDH, MDR ME POX, SOD) from six different adult tissues (brain , eye, heart, kidney, liver, muscle) of adult black carp (Mylopharyngodon piceus) were studied and the loci and phenotypes of the ten isozymes were analysed. The results showed that the α -AMY, EST, IDK LDH, MDR ME POX, SOD and G6PDH electroporesis pattern had a specific tissue distribution while the GOT had not. The physiological significance was disscussed in relation to the extersive tissue-specific distribution of the isozymes.
出处
《水生生物学报》
CAS
CSCD
北大核心
1997年第4期353-358,共6页
Acta Hydrobiologica Sinica
关键词
鱼纲
青鱼
电泳
同功酶
Isozymes
Black carp
Electrophoresis
