摘要
采用荧光光谱和紫外吸收光谱法研究了柿子单宁对牛血清白蛋白(BSA)的荧光猝灭作用。结果表明,柿子单宁可以有规律地使BSA内源荧光猝灭,其猝灭机理可认为是柿子单宁与BSA形成复合物的静态猝灭,并获得了不同温度下柿子单宁与BSA作用的结合常数和热力学参数。根据所得结果可推断柿子单宁与BSA的作用力为疏水作用力和静电作用力,同时由Forster非辐射能量转移理论计算得出了柿子单宁与BSA结合位置的距离。
The interaction between persimmon tannin and bovine serum albumin （BSA） and the mechanism of fluorescence quench were studied by fluorescence spectroscopy and ultraviolet-visible absorption spectroscopy. The results showed that the fluorescence of BSA is quenched regularly by persimmon tannin. The binding constants and thermodynamic parameters of persimmon tannin with BSA are obtained at different temperatures. The hydrophobic interaction force and electrostatic force play a main role in the binding of persimmon tannin with BSA. The distances of binding site between persimmon tannin and BSA are also obtained according to the theory of Forster＇s non-radiative energy transfer.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第6期104-107,共4页
Food Science
基金
广东省农业科技攻关计划项目(2007A020200003-2)
关键词
柿子单宁
牛血清白蛋白
相互作用
荧光猝灭
persimmon tannin
bovine serum albumin （BSA）
interaction
fluorescence quench
