粉丝类产品中转基因成分的检测

Detection of Genetically Modified Ingredient in Vermicelli

本实验采用改良的简易CTAB法从深加工产品粉丝中获得高纯度和高浓度的DNA。以CaMV35S、FMV35S、nos、nptⅡ、pat、bar6个外源基因作为筛选基因,优化反应条件和反应参数,建立了粉丝类产品中转基因成分的实时荧光PCR检测方法。该方法灵敏、特异,其推广应用对于促进粉丝类产品出口和加强转基因食品监管工作,具有重要意义。

Comparison of different kits and different methods indicates that DNA with high purity and high yield can be extracted from vermicelli by the modified CTAB method. After optimizing experimental parameters, a real time PCR assay for detecting the genetically modified ingredient in the vermicelli was developed with CaMV35S, FMV35S, nos, npt Ⅱ, pat and bar genes as detective genes. This method is proved to be sensitive and specific, which is helpful to the exportation of vermicelli and supervision of genetically modified （GM） food.