无糖和低糖食品中的糖和糖醇同时分析的阴离子交换色谱-脉冲安培检测法研究

Simultaneous Determination of Sugars and Sugar Alcohols in Sugar-free and Low-sugar Food with High Performance Anion-Exchange Using Pulsed Amperometric Detection

建立并优化了高效、简便的阴离子交换色谱-脉冲安培检测器同时分析无糖食品中的糖和糖醇的方法,并且通过样品加标回收(回收率为88%～106%)以及与其他同系列色谱柱的互补、对照实验完善了该方法并验证其准确性和可靠性。该方法共检出了14个样品中的丙二醇、肌醇、甘油、D,L-木糖醇、D-山梨醇、D-甘露醇、半乳糖醇、D-葡萄糖、D-果糖、蔗糖和麦芽糖,其分析的线性范围为0.2×10-6～40×10-6,线性相关系数为99.8%～99.9%。

High-performance anion-exchange chromatography coupled with pulsed amperometric detection was optimized for determination of sugars and sugar alcohols in sugar-free food by recovery rates （88% - 106%） of spiked samples and comparison with other type columns of CarboPac family. Determination results of propylene glycol, inositol, glycerol, D,L-xylitol, D-sorbitol, D-mannitol, galactitol, D-glucose, D-fructose, sucrose and maltose in fourteen samples are in wide linear range 0.2 × 10^-6-40 × 10^-6 with coefficients 99.8%~99.9%.