皱瘤海鞘神经腺上皮细胞活动周期与性腺发育关系的研究

RELATION OF NEURAL GLAND EPITHELIAL CELL ACTIVITY CYCLE TO GONADAL DEVELOPMENT IN STYELA PLICATA

对皱瘤海鞘神经腺上皮细胞活动周期进行了组织学、超微结构和免疫细胞化学的研究。结果表明皱瘤海鞘神经腺细胞活动周期与性腺发育相关。在性腺发育早期，神经腺各小叶上皮细胞基本上属胞质致密形式。性腺Ⅱ～Ⅲ期，有些小叶腺细胞出现空泡化，空泡化小叶占统计总数的２３％，性腺Ⅳ和Ⅴ期，增至３５％～３９％和６３％。产卵后大多数腺小叶为空泡形式，或网状形式。超微结构研究进一步证实组织学观察。免疫细胞化学定位首次证实了神经腺细胞对人促黄体素和人绒膜促性腺激素抗体发生强免疫阳性反应。为皱瘤海鞘神经腺上皮细胞可能具有内分泌功能提供了证据。

Using light and electron microscops and immunocytochemical techniques, the cycle of activity in epithelial cells of neural gland of Styela plicata were investigeted. The results indicated that the cycle of cellular activity of neural gland in styela plicata was related to gonadal development. All epithelial cells in each lobule of neural gland showed compct form of cytoplasm during early stage of gonadal development. During stage Ⅱ～Ⅲ, the glandular cells of some lobules were vacuolated, and the vaculated lobules were about 230% of the total. During stage Ⅳ and Ⅴ, the number of vacuolated lobules increased to about 35%～39% and 63%, resoectively. The great majority of the lobules in the neural gland were vacuolated or reticular form after spawning. The histological observations were verified by ultrastructural studies. Immunocytochemical localization demonstrated for the first time that the epithelial cell of neural gland in stage Ⅲ～Ⅳ showed strong immuno positive reaction to human LH and HCG antibodies. The immunoactivity of the two antibodies were similar. These results provide evidence that the epithelial cells of neural gland in Styela plicata may have endocrine function. Therefore, the present study has important significance in theory for understanding the evolutive position of the reproductive endocrine in urochordata.