曼氏血吸虫童虫表膜12KD抗原分子的提取和氨基酸顺序分析

PURIFICATION AND PROTEIN SEQUENCING OF A 12KD MOLECULE ON THE SCHISTOSOMULUM TEGUMENTS OF SCHISTOSOMA MANSONI

应用ＴｒｉｔｏｎＸ－１１４相分离技术提取曼氏血吸虫３小时龄童虫的整个表膜蛋白质，并经免疫亲和层析提取与放射致弱尾蚴免疫家兔血清ＩｇＧ（ＶＲＳ－ＩｇＧ）结合的童虫表膜抗原。在Ｗｅｓｔｅｒｎｂｌｏｔ分析中，经ＶＲＳ识别，纯化童虫表膜抗原出现６条主要区带，其分子量从高到低依次为７２、５５、３１、２８、１６和１２ＫＤ。选择分子量为１２ＫＤ的抗原分子进行氨基酸顺序分析，得到一段含３６个氨基酸残基的序列。

The integral membrane proteins of 3 hour schistosomula of S.mnsoni were extracted using Triton X 114 phase separation.By immunoaffinity column coupling with the vaccinated rabbit serum IgG (VRS IgG),the antigens of the integral membrane proteins were purified.The western blotting profiles of the purified membrane antigens consisted of at least 6 bands,including 72,55,31,28,16 and 12KD molecules.The 12KD molecule was selected to be subjected to protein sequencing.A sequence of 36 amino acid residues was obtained.