丹皮酚对急性微循环障碍大鼠血管内皮细胞分泌的ET-1和NO的调控

Detection of ET-1 and NO secretion from vascular endothelial cell in acute microcirculation damaged SD rat and the effects of Paeonol

目的研究丹皮酚对血管内皮的保护作用及机制。方法主要通过体内实验研究,用肾上腺素和冰水复制大鼠的血管内皮损伤模型,用CD34标记血管内皮细胞,观察丹皮酚对血管内皮形态学的影响,酶联免疫吸附实验(ELISA)检测血清中内皮素-1(ET-1)和一氧化氮(NO)。结果肾上腺素和冰水造成大鼠血管内皮损伤,主要表现为管腔扩张,血栓形成,内皮细胞收缩,脱落,而丹皮酚给予组能够明显抑制血栓形成,防止内皮细胞脱落。造模后血清中ET-1分泌增加,而NO分泌明显减少。丹皮酚能够抑制ET-1的分泌而促进NO的分泌。结论丹皮酚具有血管内皮保护作用,可以预防物理造成的血管内皮的损伤,主要通过调节ET-1和NO的平衡。

OBJECTIVE To detect the effects of Paeonol on the function of vascular endothelial cells.METHODS The sprague-dawley rats were established by injected with adrenaline, irritated into cold-water. To detect the ET-1 in the serum through enzyme immunoassay （EIA） and to detect the NO through nitrate reductase, to localize the vascular endothelial cells by Immunohistochemistry. RESULTS Immunohistochemistry showed that in the model group, vessel contracted, area of vessel shrank, some vascular endothelial cells dropped into lumen, obstructed the lumen together with some red blood cells, vascular endothelial membrane lost integrality. There were no great signs showing damaged vascular endothelial cells in the treatment with paeonol. The secretion of ET-1 was higher in model rats than control, while the secretion of NO was lower in model rats than control.Paeonol inhibited the secretion of ET-1 and upregulated the secretion of NO. CONCLUSION Paeonol can protect the dysfunction of VECs caused by extremely contraction and dilation; inhibited the secretion of ET-1 and upregulated the secretion of NO.