对氯烯雌酚醚防治雌性大鼠去势后骨丢失的评价

Prevention of Bone Loss by Chlorotrianisene in Oophorectomized Rats

目的：了解氯烯雌酚醚对去势雌性大鼠骨代谢的影响。方法：鼠龄７０天的Ｗｉｓｔａｒ雌鼠４８只，分别予假性手术与注射用油（ＳＶ组）、去势与注射用油（ＯＶ组）、假性手术与氯烯雌酚醚（ＳＥ组）及去势与氯烯雌酚醚（ＯＥ组）等处理，双侧卵巢去势或假性手术后７天，于每晚腹腔注射氯烯雌酚醚或注射用油４ｍｌ／ｋｇ，共４５天。处死大鼠时，测定子宫湿重，同时收集第１２胸椎和左胫骨制成脱钙骨切片，行骨组织形态计量学测定。结果：４组间子宫重量差异均有显著性。骨组织形态计量学显示，（１）ＯＶ与ＳＶ、ＳＥ及ＯＥ组间差异有显著性；（２）ＯＥ、ＳＥ和ＳＶ组间差异无显著性。结论：氯烯雌酚醚能抑制Ｗｉｓｔａｒ雌鼠去势后的骨丢失，减缓去势后的子宫萎缩，对骨骼有雌激素样的保护作用。

Objective: To determine the influence of chlorotrianisene on bone metabolism in oophorectomized rats. Methods: Forty eight Wistar female rats, 70 days of age, were randomly divided into four groups: SV group (sham operation+vehicle), OV group (oophorectomy+vehicle), SE group (sham operation+chlorotrianisene) and OE group (oophorectomy+chlorotrianisene). Chlorotrianisene or vehicle 4 ml/kg was given i.p. daily for 45 days from day 7 of being ovariectomized or sham operated. At the time of death, uterine weight was measured, and the thoracic vetebra 12, left tibia were collected and made into decalcified bone specimens for histomorphometry. Results: There were significant difference in the uterine weight among each group. Significant different appearance by bone histomorphometry was seen between OV and the other three groups, but not shown between SV and SE, OE groups. Conclusion: These results suggest chlorotrianisene could inhibit bone loss and delay the atrophy of uterus induced by ovariectomy in Wistar female rats.It has protective effects on bone like other estrogen preparations.