摘要
目的探讨谷氨酸(Glu)对大鼠下丘脑弓状核(ARC)神经元电活动的影响。方法采用电生理学方法,细胞外记录大鼠离体下丘脑内侧基底部ARC神经元的电活动,观察Glu及N-甲基-D-门冬氨酸受体激动剂NMDA对ARC神经元放电的影响。结果Glu(200μmol/L)可使ARC神经元电活动增强,ARC神经元放电频率由给药前的(1.26±0.31)Hz增加至(1.78±0.59)Hz;NMDA(25μmol/L)也可使ARC神经元电活动增强,ARC神经元放电频率由给药前的(1.27±0.22)Hz增加至(2.36±0.68)Hz;NMDA受体拮抗剂MK-801(100μmol/L)能阻断Glu、NMDA引起的ARC神经元放电频率加快效应。结论Glu、NMDA均可显著增强ARC神经元电活动,在下丘脑弓状核神经元电活动的调节中有Glu机制的参与。
Objective To study the effect of glutamic acid（Glu） on the electric activities of arcuate nucleus neurons （ARC） in rats. Methods The experiment was performed on isolated mediobasal hypothalamus to observe the influence of Glu and N-methyl-D-aspartate（NMDA） receptor agonist NMDA on the unit discharges of ARC neurons by extracellular recordings in vitro. Results After administration of Glu （200 μmol/L） the discharge frequency of ARC neurons was increased significantly from （1.26±0.31） Hz before Glu administration to （1.78±0.59） Hz. The discharge frequency of ARC neurons was also increased significantly from （1.27±0.22） Hz to （2.36±0.68） Hz after NMDA （25 μmol/L） administration. These effects were blocked by MK-801（100 μmol/L）, NMDA receptor antagonist. Conclusion Both Glu and NMDA increase the discharge frequency of ARC neurons significantly. Glutamate mechanism might be involved in the modulation of the electric activities of hypothalamic arcuate nucleus neurons.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2008年第3期379-382,共4页
Suzhou University Journal of Medical Science
