摘要
目的研究胰腺癌组织中缺氧诱导因子2(HIF-2α)的表达及其与血管内皮生长因子(VEGF)、微血管密度(MVD)的关系,分析胰腺癌中HIF-2α表达促进肿瘤血管生成的作用及与临床病理特征之间的关系。方法应用免疫组化SP法检测60例胰腺癌及相应正常胰腺组织中HIF-2α、VEGF蛋白的表达和分布,计数MVD,分析其间的相关性及其与肿瘤临床病理特征之间的关系。结果HIF-2α和VEGF在胰腺癌组织中的表达均明显高于正常胰腺组织,胰腺癌组织中MVD值明显高于正常胰腺组织,差异均有统计学意义(P<0.05)。HIF-2α的表达与VEGF的表达及MVD值成正相关(P<0.05),HIF-2α与VEGF的表达与胰腺癌TNM分期、肿瘤大小有关。结论胰腺癌组织中HIF-2α呈过量表达,并与VEGF及MVD显著相关;它可能通过上调VEGF表达促进胰腺癌血管生成,进而影响胰腺癌的发生、发展。
Objective To investigate the expression of hypoxia-inducible factor 2α （HIF-2α） and its relationships with vascular endothelial growth factor （VEGF） and microvessel density（MVD） in angiogenesis,and the relationship to the clinicopathologic features of human pancreatic carcinoma. Methods The expressions of HIF-2α and VEGF as well as the value of MVD were detected by immunohistochemical method of SP in 60 cases of resected specimen of pancreatic carcinoma and their corresponding normal pancreatic tissue served as the control group. The correlations among them,and their relationships to the clinicopathologic characteristics of human pancreatic carcinoma were analyzed. Results The positive expressions of HIF-2α and VEGF in pancreatic carcinoma were significantly higher than that in the control group （P〈0.05）.The value of MVD was also significantly higher than that in the control group（P〈0.05）. The expression of HIF-2α was positively correlated with the expression of VEGF and the value of MVD closely（P〈0.05）.The positivity rates of HIF-2α and VEGF were closely related with TNM staging and tumor size of human pancreatic carcinoma respectively. Conclusion HIF-2α is overexpressed in pancreatic carcinoma,and its expression is closely correlated with the expression of VEGF and the value of MVD.It may be involved in the angiogenesis of human pancreatic carcinoma by upregulating the expression of VEGF,and play an important role in the carcinogenesis and aggression of pancreatic carcinoma.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2008年第3期423-425,429,F0003,共5页
Suzhou University Journal of Medical Science
基金
卫生部科学研究基金资助项目(WKJ2004-2-011)
