截肢后大鼠血浆及肝肾组织中硫化氢/胱硫醚-γ-裂解酶含量的变化

Changes of hydrogen sulfide (H2S)/cystathionine-γ-lyase(CSE) content in plasma、liver and kidney of rats with amputation

目的探讨截肢后大鼠血浆及肝、肾等远隔器官组织中内源性硫化氢(hydrogensulfide,H2S)的含量变化规律,以期找到硫化氢在创伤与修复过程中的作用机制。方法通过手术制备大鼠左后肢截肢模型,将雄性Wistar大鼠按手术时间随机分为9组:正常对照组(7只);手术后1、2、4、6、12、24、48、72h组(各7只)。测定血浆中H2S含量及肝、肾组织中CSE活性,分析其随时间变化规律。光镜观察各组组织形态学变化。结果与正常对照组相比,手术后大鼠血浆H2S含量逐渐降低,6h达最低值39.286±6.526(P<0.01),此后逐渐升高,48h后基本恢复正常。肝、肾组织CSE活性变化大体趋势与血浆相同,但下降幅度及到达最低值时间不同,肝在术后12h最低为166.162±10.878(P<0.01),肾在术后6h最低为60.664±7.574(P<0.01)。肝、肾组织出现一定程度组织损伤。结论H2S是一种新的内源性介质,在体内广泛存在,可能参与了组织损伤与修复的一系列病理生理过程。

Objective To investigate the changes and significance of hydrogen sulfide （H2S）level in both plasma and various tissues, including liver,kidney,in rats with amputation. Methods A rat model of amputation was developed. Male Wistar rats were divided into 9 groups at random：Group normal（C,7）,Group postop 1h,2h,4h,th, 12h,24h,48h,72h（B,7）. The plasma contents of H2S were detected, so did the CSE activity in liver and kidney. The structures of various tissues were observed. Results Amputation to male Wistar rats caused a significant decrease in H2S contents in plasma as well as liver,kidney within 12 hours and recuperated at 48h（P〈0.05 respectively）. The plasma levels of H2S decreased to the lowest at 6h,the valley bottom of CSE activity in liver was at 12h,but in kidney it was at 6h. The tissue injury of liver and kidney emerged in the postoperative groups. Conclusions H2S may be a new endogenous mediator and play a significant role in the process of tissue injury and repair.