期刊文献+

寄生加勒比松松材线虫的鉴定及其致病性测定 被引量:3

Identification of Bursaphelenchus xylophilus from Pinus caribaea and Pathogenicity Assay
下载PDF
导出
摘要 运用线虫比较形态学和rDNA-ITS-PCR-RFLP方法对采自广东省广州市白云山加勒比松上的伞滑刃线虫进行鉴定,结果确定该线虫为松材线虫。致病性测定表明该松材线虫种群对加勒比松苗有较强的致病力,接种的松苗均出现不同程度的枯萎,而且从枯死木中可重新分离到该线虫。 Bursaphelenchus xylophilus, collected from Pinus caribaea in Guangzhou city, Guangdong province, was confirmed by morphology and rDNA-ITS-PCR-RFLP. Host seedlings of P. caribaea were inoculated with the isolated nematodes. The nematodes displayed pathogenicity in the host seedlings. All seedlings inoculated with the nematodes wilted and B. xylophilus could be recovered from the seedlings.
出处 《广东林业科技》 2008年第2期37-41,共5页 Forestry Science and Technology of Guangdong Province
基金 广东省科技计划项目(2004B20501005)资助。
关键词 松材线虫 分子鉴定 加勒比松 致病性 Bursaphelenchus xylophilus, molecular identification, Pinus caribaea, pathogenicity
  • 相关文献

参考文献15

  • 1Ryss A, Vieira P, Mota M, et al. A synopsis of the genus Bursaphelenchus Fuchs, 1937 (Aphelenchida: Parasitaphelenchidae) with keys to species [ J]. Nematology,2005,7:393-458.
  • 2OEPP/EPPO. EPPO standards PM 7/4 ( 1 ) diagnositic protocols for regulated pests Bursaphelenchus xylophilus [ J ]. Bulletin OEPP/EPPO Bulletin, 2001, 31: 61-69.
  • 3Liao J L, Zhang L H, Feng Z X. Reliable identification of Bursaphelenchus xylophilus by rDNA amplification [ J ]. Nematologia Mediterranea, 2001, 29 : 131-135.
  • 4Ferris V R, Ferris J M, Faghihi J. Variation in spacer ribosomal DNA in some cyst - forming species of plant parasitic nematodes[ J ]. Fundamental and Applied Nematology, 1993,16 : 177-184.
  • 5Vrain T C. Restriction fragment length polymorphism separates species of the Xiphinema americanus group [J]. Journal of Nematology, 1993, 25: 361-364.
  • 6Mamiya Y, Kiyohara T. Description of Bursaphelenchus lignicolus n. sp. from pine wood and histopathology of nematode - infested trees [ J]. Nematologica, 1972,18 : 120-124.
  • 7Burgermeister W, Metge K, Braasch H, et al. ITS-RFLP patterns for differentiation of 26 Bursaphelenchus species (Nematoda: Parasitaphelenchidae) and observations on their distribution[ J]. Russian Journal of Nematology,2005,13 ( 1 ) :29-42.
  • 8Ye W M, Giblin - Davis R M, Braasch H, et al. Phylogenetic relationships among Bursaphelenchus species ( Nematoda : Parasitaphelenchidae) inferred from nuclear ribosomal and mitochondrial DNA sequence data[ J]. Molecular Phylogenetics and Evolution, 2007, 43: 1185-1197.
  • 9Zheng J W, Subbotin S A, He S S, et al. Molecular characterisation of some Asian isolates of Bursaphelenchus xylophilus and B. mucronatus using PCR-RFLPs and sequences of ribosomal DNA [J]. Russian Journal of Nematology,2002,11 (2) :17-22.
  • 10Kanzaki N, Futai K. A PCR primer set for determination of phylogenetic relationships of Bursaphelenchus species within the xylophilus group[ J]. Nematology, 2002, 4( 1 ) : 35-41.

二级参考文献9

  • 1王宏毅,杨再福,张绍升.日本针叶木伪伞滑刃线虫鉴定及其与近似种的比较[J].福建农林大学学报(自然科学版),2004,33(1):34-37. 被引量:9
  • 2Kanzaki N,Tsuda K,Futai K.Description of Bursaphelenchus conicaudatus n.sp.(Nematoda:Aphelenchoididae),isolated from the yellow-spotted longicorn beetle,Psacothea hilaris(Coleoptera:Cerambycidae) and fig tree,Ficus carica[J].Nematology,2000,2(2):165-168.
  • 3Braasch H,Gu J,Bugermeister W,et al.Bursaphelenchus doui sp.n.(Nematoda:Parasitaphelenchidae)in packaging wood from Singaporea new species of the B.xylophilus group[J].Russian Journal of Nematology,2004,12(2):19-27.
  • 4Kanzaki N,Tsuda K,Futai K.Phylogenetic analysis of the phoretic association between Bursaphelenchus conicaudatus (Nematoda:Aphelenchoididae) and Psacothea hilaris(Coleoptera:Cerambycidae)[J].Nematology,2002,4(6):759-771.
  • 5Gu J,Zhang J,Braasch H,et al.Bursaphelenchus singaporensis sp.n.(Nematoda:Parasitaphelenchidae) in packaging wood from Singaporea new species of the B.xylophilus group[J].ZOOTAXA,2005(988):1-12.
  • 6Bugermeister W,Gu J,Braasch H,et al.Bursaphelenchus arthuri sp.n.(Nematoda:Parasitaphelenchidae)in packaging wood from Taiwan and South Koreaa new species belonging to the fungivorus group[J].J Nem Morph Syst,2005,8(1):7-17.
  • 7Braasch H, Jianfeng Gu, Burgermeister W, et al. Bursaphelenchus doui sp, n. (Nematoda: Parasitaphelenchidae) in packaging wood from Singapore-a new species of the B. xylophilus group [J]. Russian Journal of Nematology, 2004. 12 (2) : 19 - 27.
  • 8Kanzaki N, Tsuda K, Futai K. Description of Bursaphelenchus conicaudatus n, sp. (Nematoda: Aphelenchoididae), isolated from the yellow-spotted longicorn beetle, Psacothea hilaris (Coleoptera: Cerambycidae) and fig tree, Ficus carica [J]. Nematology, 2000, 2 (2): 165- 168.
  • 9Jianfeng Gu, Jiancheng Zhang, Braasch H, et al. Bursaphelenchus singaporensis sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Singapore-a new species of the B. xylophilus group [J]. ZOOTAXA, 2005, 988:1 - 12.

共引文献3

同被引文献76

引证文献3

二级引证文献64

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部