体外压力刺激对大鼠筋膜组织细胞合成释放NO和IL-1β影响的研究

Study on synthesis and liberation of NO and IL-1β from fascia tissue cells of rat under pressure stimulation in vitro

目的:通过探讨压力刺激对大鼠筋膜组织细胞合成释放一氧化氮(NO)和白细胞介素-1 β(IL-1 β)的影响,为按摩治病机制提供细胞生物力学方面的实验依据。方法:体外培养大鼠筋膜组织细胞,对细胞进行形态学鉴定后,实施50kPa压力刺激,检测细胞外培养液中NO和IL-1 β含量的变化。结果:筋膜组织细胞主要是成纤维细胞,压力作用后,细胞外培养液中NO和IL-1 β的含量都增加,差异均有统计学意义(P<0.05)。结论:筋膜组织细胞可以感受机械刺激,并将物理性质的力学信号转换为生物性质的化学信号;其在力学刺激作用下的反应,可能是按摩发挥治病作用的细胞生物力学原理之一。

Objective： To provide some experimental basis of cell biomechanics for clinic treatment of massage by exploring synthesis and liberation of NO and IL-1β from fascia tissue cells of rat under pressure stimulation in vitro. Methods： Fascia tissue cells of rat were cultured in vitro. After morphological identification ,50kPa pressure was forced on these cells, then the contents of NO and IL-1β in culture medium were detected. Results： The fascia tissue cells are mainly made up of fibroblast cells. The contents of NO and IL-1β in culture medium were both increased significantly under pressure stress. Conclusion： Fascia tissue cells can receive the mechanical stimulation, and transform the mechanical signals into biological signals. The reaction to mechanical stimulation may be one of the cell biomechanical principles of massage.