摘要
S-腺苷甲硫氨酸合成酶（s-adenosylmethionine synthetase SAMS）是植物代谢中的一个关键酶，它催化甲硫氨酸与ATP生物合成S-腺苷甲硫氨酸（SAM）。SAM是生物合成乙烯以及多胺的前体，参与了植物的转甲基、转氨丙基、转硫反应等多种重要的生理过程。Frank等研究发现，SAM可以与RNA结合参与基因表达调控。近年来研究表明，乙烯和多胺都参与了植物抗逆反应，
[Objective] The mRNA expression level changes of S-adenosylmethionine synthetase （SAMS） under low temperature stress was studied. [Method] Total RNA were extracted from leaves, stem and earthnut of sweet potato 0,12,24,48 and 72 h after low temperature treatement, mRNA expression level was analyzed by reverse expression and Real-time PCR technique. [Result] The expression quality of the gene extracted from leaves, stem and earthnut increased and the expression quality reached the peak point 24,72 and 72 h after low temperature treatment respectively. The expression change of earthnut was the biggest. [Conclusion] Low temperature was good for increasing mRNA expression of relevart genes.
关键词
甘薯
S-腺苷甲硫氨酸合成酶
MRNA
基因表达
Low temperature stress
Ipomoea batats L
S-adenosyl methionine synthetase
Real-time PCR
