摘要
RT-PCR作为一种现代分子生物学基因诊断技术，具有高度敏感性和特异性，能在数小时内检出痕量病原，可克服传统的禽流感病毒（AIV）诊断技术病毒分离鉴定试验周期长的缺点，为AIV早期快速诊断提供了敏感、快速、实用的方法。引物设计是保证RT-PCR成功的重要前提。笔者对运用Primer Premier5.0软件设计H5和H7亚型AIV的多重RT-PCR引物进行了探讨。
[Objective] The research aimed to design primers that are suitable for detecting H5 and H7 subtypes of avian influenza virus （AIV） ; [Method] DNAStar was used to analyze the homology of the sequences of H5 and H7 subtypes of AIV accessed in GenBank, and design primers（ by Primer Premier 5.0） on high homologous region of these sequences, and then amplified by RT-PCR. [Result] The multiplex RT-PCR amplification, agarose gel electrophoresis and sequencing results showed that the self-designed primers are successful for detecting AIV. [Conclusion] It is feasible to rapidly diagnose AIV through this method.
基金
Supported by Important Project of Jinlin Provincial Science and Technology Department(20065020)~~
关键词
禽流感病毒
RT-PCR
生物学
基因诊断
Avian influenza virus
Primer Premier 5.0
DNAStar
Multiplex RT-PCR amplification
