摘要
利用现代生物技术组织培养方法对野百合进行种质保存和快速繁殖研究，对发展野百合的大规模生产及百合育种工作，保护野生资源和生态环境有重要意义。
With different parts of bulb scale as explants, the proliferation method of Guizhou Lilium brownii were studied with 3% sodium hypochlorite and MS medium with different concentrations of hormones. The results show that it is feasible to disinfect the bulbs of Lilium brownii with 3% sodium hypoehlorite, moreover, the sodium hypochlorite is very cheap and harmless to researchers, experimental materials and environment. MS ＋ NAA 0.3 mg/L ＋ 6-BA 1.5 mg/L is optimum for the induction of bulbs and, the basal part of Lilium brownie is the optimum explants. After culture for 25 d on the same medium, the tube bulbs could be obtained with the characteristics of high propagation coefficient, strong and new roots. The survival rate is over 90% for transplantation of tube bulbs with diameter between 1-2cm. The method developed in the present study can proliferate abundant Lilium brownii seedling in short time.
基金
Supported by the Nomarch Funds for Excellent Science and Technology Teachers of Guizhou Province(S2004-17)
the Special Foundation for Im-proving Scientific Research Condition of Guizhou Province(Q2005-4)
the Doctor Startup of Guiyang Medical College(C2005-6)~~
