玉米叶片DNA快速提取方法改进研究

An Improved Study on Rapid DNA Extraction Method from Young Leaves of Maize

分子生物学研究需要大量的DNA样品,DNA的快速提取是提高研究效率的关键。研究比较多种植物DNA的提取方法,对部分实验步骤做了修改,建立了适于玉米叶片DNA微量快速提取的新方法。该方法不仅操作步骤简单、速度快、省时间,而且可以确保提取的玉米基因组DNA有足够的浓度和纯度。利用此方法,在一般实验室条件下每人每天可以提取150～200个DNA样品,一个DNA样品可供50～60次PCR反应使用,DNA质量可以确保一般的PCR扩增,适用于玉米遗传多样性、分子标记辅助选择、引物筛选和分子标记定位等多种研究目的。

A new method of rapid simple DNA extraction from maize was established based on CTAB procedure. Compared with CTAB procedure, this method is superior of using fewer reagents, simpler, faster and costs-saving. The extracted DNA was suitable for SSR analysis. More than 150 DNA samples, could be extracted a day by one person with this method. It indicates that the method can be used in maize genetic diversity, molecule marker assistant selection, primer screening, and QTL mapping with SSR markers.