摘要
目的探讨谷胱甘肽耗竭与微囊藻毒素-LR(MCLR)细胞毒性的关系。方法不同剂量的MCLR作用人肝癌细胞HepG2后,采用四甲基偶氮噻唑蓝法检测细胞毒性,并用二硫硝基苯甲酸法测定细胞内还原型谷胱甘肽(GSH)的含量。结果细胞存活率随处理剂量的增加而降低,抗氧化剂N-乙酰半胱氨酸明显减轻MCLR诱导的细胞毒性。细胞内GSH含量显著降低,处理剂量与GSH水平之间呈现剂量-反应和时间-反应关系。结论谷胱甘肽耗竭可能是微囊藻毒素-LR引起细胞毒性的原因之一。
Objective To study the relationship between glutathione depletion and the cytotoxicity of microcystin- LR（MCLR）. Methods Human hepatoma cell lines HepG2 were treated with MCLR, then the cytotoxicity was measured by the MTT assay and the level of intracellular glutathione （GSH） was measured by the colofimetfic 5,5＇ - dithio - bis （2- nitrobenzoic acid） （DTNB） assay. Results MCLR dose - dependently decreased the cell viability, which were inhibited by N - acetyl - cysteine. In MCLR - treated HepG2 cells, GSH levels decreased in a dose - and time - dependent manner. Conclusion Glutathione depletion is playing important roles in MCLR- induced cytotoxicity.
出处
《应用预防医学》
2008年第3期129-131,共3页
Applied Preventive Medicine
基金
国家留学基金委资助项目(No.20845014)
