类风关巴布剂穴位贴敷对胶原诱导关节炎大鼠滑膜细胞核转录因子-κ β表达的影响

Effect of Rheumatoid Arthritis Cata-plasma Acupoint Application on Synovial Cell Nuclear Factor-κ β Expression of CIA Rats

目的比较不同组合穴位贴敷类风关巴布剂对牛Ⅱ型胶原诱导关节炎(CIA)大鼠滑膜细胞核转录因子-κβ(NF-κβ)表达的影响。方法大鼠背部5处皮内注射牛Ⅱ型胶原诱导大鼠关节炎,观察以雷公藤为主的中药类风关巴布剂不同组合穴位贴敷对模型大鼠免疫组化指标NF-κβ计分,以及滑膜细胞上清液的白细胞介素-1β(IL-1β)与肿瘤坏死因子-α(TNF-α)的影响。结果类风关巴布剂治疗后,各穴位贴敷组大鼠的NF-κβ计分较模型组降低,其中系统组(远近配穴)最接近正常组;各组滑膜浸液中IL-1β、TNF-α浓度降低。结论类风关巴布剂穴位贴敷能够降低CIA大鼠滑膜细胞的NF-κβ的蛋白表达,并降低相关细胞因子的浓度,抑制正反馈效应;远近配穴可能通过标本兼治,多靶点起效,使其整体疗效优于单纯远道取穴或局部取穴。

Objective To compare the rapeutic effects of different acupoint combination applied with Rheumatoid Arthritis Cam-plasma on nuclear factor-κβ expression of CIA rats. Methods Induced arthritis rats back were endermic injected with type Ⅱ bovine collogen as animal models. There were five groups for comparison. NF-κβ, interleukin-1β（IL-1β） and tumor necrosis factor-α （TNF-α） of synovial cell clear supematant liquid were observed. Results NF-κβ, IL-1β and TNF-α scores in therapy groups were steped down. The scores of systematic selection （distant and near coordination） of acupoints was the most close to normal group. Conclusion Rheumatoid Arthritis Cata-plasma can down-regulation the expression of nuclear factor-κβ and correlated cell factor density of CIA rats synovial cell and repress positive feedback. Therapeutic effect of distant and near coordination of acupoints is better than distant selection and local selection of acupoints, probably by treating appearance and substance simultaneously to multitarget effect.