HPLC法测定复方益肝灵片中水飞蓟宾的含量

Determination of Silybin in Fufang Yiganling Tablet by HPLC

目的建立复方益肝灵片中水飞蓟宾的含量测定方法。方法采用HPLC法;流动相:甲醇-水-冰醋酸(48∶52∶1);检测波长:286nm。结果水飞蓟宾在0.2380～2.380μg范围内线性关系良好,其回归方程为:Y=2.146×106X+1.818×103,r=0.9999,平均加样回收率为98.17%,RSD=1.3%。结论该方法简单、准确、重复性好,可作为复方益肝灵片的质量控制方法。

Objective To establish a method for the determination of silybin in Fufang Yiganling tablet. Methods RP-HPLC was used and the mobile phase was consisted of methanol-water-glacial acetic acid （48 ： 52 ： 1）. The detection wavelength was 286 nm. Results The calibration curve of Silybin was linear in the range of 0.238 0-2.380 μg, linear equation： Y=2.146×10^6X＋1.818×10^3, r=0.9999, with average recovery of 98.17% （RSD=1.3%）. Conclusions The method is simple, accurate and with good reproducibility. It can be used to control the quality of silybin in Fufang Yiganling tablet.