反相高效液相色谱法同时测定车前子中的毛蕊花苷和异毛蕊花苷

Determination of Verbascoside and Isoverbascoside in Semen plantaginis by RP-HPLC

采用反相高效液相色谱法在Waters Symmitry C18 （5 μm，250 mm×4.6 mm）色谱柱上以甲醇：体积分数0.1%甲酸溶液（40：60，v：v）为流动相同时测定车前子中毛蕊花苷和异毛蕊花苷的含量，流动相流速为0.6 mL/min，检测波长为330 nm。毛蕊花苷线性范围为0.2～2.0 μg，相关系数R＝0.9982。异毛蕊花苷线性范围为0.2～2.0 μg，R＝0.9957。样品的平均回收率分别为99.70 %，101.18%。测得大粒车前子中毛蕊花苷含量为8.65 mg/g，异毛蕊花苷含量为1.36 mg/g。此方法准确、快速，适用于车前子中毛蕊花苷和异毛蕊花苷的定量分析。

A method for determining verbascoside and isoverbascoside in Semen plantaginis by RP-HPLC was described. The determination was carried out on a Waters Syrnmitry C18 （5 μm, 250 mm × 4.6 mm） column at 25 ℃. 0.1% HCOOH：MeOH （60：40, v：v） was used as mobile phase at a flow rate of 0.6 mL/min and detection wavelength was set at 330 ran. The linearity of verbascoside was found in the range of 0.2-2.0 μg,r = 0. 9982 ,the average recovery rate was 99.70%. The linearity of isoverbascoside was found in the range of 0.2-2.0 μg,r = 0. 9957, and the average recovery rate was 101.18%. The contents of verbascoside and isoverbascoside in Semen plantaginis were determined as 8. 65 mg/ g and 1.36 mg/g,respectively. This method is reliable,accurate and suitable for the determination of verbascoside and isoverbascoside in Semen plantaginis.