NDV F48E9株与LaSota株HN结构蛋白潜在抗原表位的预测分析

Prediction of latent antigenic epitopes for NDV F48E9 and LaSota HN structure protein

该文阐述了以新城疫病毒F48E9株和LaSota株HN结构蛋白的氨基酸序列为依据,先采用Chou-Fasman方法、Garnier-Robson方法、Emini方法、Kyte-Doolittle方法和Karplus-Schulz方法分别预测HN蛋白的二级结构、表面可能性、亲水性和柔韧性,单参数分析HN蛋白的结构和性质,再通过Jameson-Wolf方法,综合不同的参数评价HN蛋白各个部位的抗原性,预测分析结果表明,将各参数综合考虑,能显著提高预测准确度.

This paper described on the basis of amino acid sequences of NDV F48E9 and LaSota HN structure protein, it takes the methods of Chou-Fasman, Garnier-Robson, Emini, Kyte-Doolittle and Karplus-Schulz to predict the secondary structure, superficial possibility, hydrophilicity and flexibility of HN protein, to analyze of the structure and nature of HN protein in single parameter;and then, it takes the method of Jameson-Wolf to evaluate comprehensively the antigenicity of HN protein. The results indicated that the accuracy of prediction can be improved remarkably by taking all parameters into account.