生存素基因mRNA和蛋白在胃癌中表达及其意义

Expression of survivin mRNA and protein in gastric carcinoma

目的探讨生存素(survivin)基因mRNA和蛋白表达与胃癌发生发展的关系。方法应用原位杂交和免疫组化技术检测30例胃癌及20例良性胃组织(5例慢性胃炎、5例胃溃疡、10例癌旁正常组织)标本中survivin mRNA和蛋白的表达,并分析其与临床病理参数的关系。原位末端标记技术(TUNEL法)检测细胞凋亡。结果良性胃组织中无survivin的表达,而胃癌组织中survivin mRNA阳性表达率36.67%(P<0.01),蛋白阳性率46.67%(P<0.01),并且其表达在不同的TNM分期、有无淋巴结转移之间比较差异有统计学意义(P<0.05)。结论survivin在胃癌中显著表达,并明显抑制凋亡,在胃癌的发生发展过程中起重要作用。

Objective To investigate the relation of survivin gene with the progress of gastric carcinoma. Methods Expression of survivin mRNA and protein was detected by in situ hibridization and immunohistochemistry in 30 cases of gastric carcinoma, 20 cases of benign gastric tissue （including 5 of chronic gastritis, 5 of gastric ulcer, 10 of neighboring noncancerous tissues）. The apoptosis in situ gastric tissue was identified by the terminal deoxynucleotidy transfer mediated dUTP-biotin nick end labeling （TUNEL）. Results There was no positive signal of survivin mRNA and protein detected in non-carcinoma of gastric tissue. In gastric carcinoma, the positive expression rate of survivin mRNA was 36.67 % and survivin protein was 46.67 %. The expression of survivin in different TNM clinical staging and with or without lymph node metastasis cases had significant difference （P〈0. 05）. Conclusion survivin is over expressed in gastric caicinoma and obviously inhibited apoptosis, survivin plays an important role in the carcinogenesis of gastric carcinoma.