流动注射化学发光分析法测定利福平

Flow injection chemiluminescence determination of rifampicin

在酸性条件下,KMnO4能氧化利福平产生弱化学发光,并且,利福平能极大的增敏Na2SO3-KMnO4体系的化学发光信号。基于此,结合流动注射技术,建立了测定利福平的新方法。在优化的条件下,利福平在5.0×10-8g/mL^1.0×10-5g/mL范围内与化学发光强度呈良好的线性关系,检出限为3×10-8g/mL。对2.0×10-6g/mL的利福平进行11次平行测定,相对标准偏差为2.6%。该法用于胶囊和滴眼液中利福平的测定,并初步探讨了该化学发光反应机理。

It was found that the rifampicin can be oxidized by acidic potassium permanganate with weak chemiluminescence（CL） emission. Moreover, rifampicin can greatly enhance the CL intensity of the system of sodium sulfite with potassium permanganate. Based on these; a novel sensitive flow injection chemiluminescence （FI-CL） method for the determination of rifampicin in pharmaceutical preparation is presented. Various factors affecting the CL emission intensity of the system have been investigated carefully. The enhancement of CL emission intensity is linear with the concentration of rifampicin in the range of 5.0 × 10^-8 g/mL - 1.0 × 10^-5 g/mL. The limit of detection is 3 × 10^-8 g/mL （3σ）. The relative standard deviation is 2.6% for 2.0 × 10^-6 g/mL rifampicin solution in eleven replicate measurements. It has been successfully used for the determination of rifampicin in pharmaceutical preparation. Moreover the CL mechanism has also been discussed.