高效液相色谱法测定胭脂红酸的研究

Study on determination of carminic acid by high performance liquid chromatography

研究了以反相C18柱，V（甲醇）：V（乙腈）：V（pH2．8H3PO4）=12：8：80混合溶液为流动相，对胭脂红酸进行高效液相色谱分析的方法。胭脂红酸在2．5—40μg／mL范围内的吸光度线性相关系数达0．9998，方法回收率在98．2％～101．1％，相对标准偏差为0．78％～1．2％，已用于样品中胭脂红酸的测定。

The RP-HPLC method was used to analyze carminic acid using a C18 reversed-phase column with acetonitrile-methanol-pH 2.8 phosphate buffer （ 12 ： 8 ： 80） as mobile phase at a flow-rate of 0.6 mL/min. The relationship between peak area and concentration of carminic acid in range of 2.5 - 40 μg/mL was linear with correlation coefficient r of 0.9998. The recoveries of carminic acid are 98.2% - 101.1%, and the relative standard devitions are 0.78%- 1.2 %. This method was applied to the determination of canninic acid in samples with good results.