血吸虫病与大肠癌关系的流式细胞术研究

Study on the Relationship of Schistosomiasis toColorectal Cancer by Flow Cytometry

目的:血吸虫病是否为大肠癌的一种癌前病变多年来一直存在不同意见,本文探讨血吸虫病与大肠癌的关系.方法:应用流式细胞术检测大肠慢性血吸虫病及大肠癌细胞DNA含量及两种癌基因表达产物(p21~ras,p^53蛋白)的变化.结果:42例大肠慢性血吸虫病患者(包括24例单纯血吸虫病和18例伴虫卵息肉者)的细胞DNA含量及两种癌基因产物表达均无明显异常,而12例伴血吸虫病的大肠癌与11例单纯大肠癌及其癌周粘膜在以上两方面亦未显示出明显差异.结论:本组资料未能表明慢性血吸虫病肠道病变作为癌前病变可能具有的恶性转化特性,从而不支持血吸虫病与大肠癌之间的直接因果联系,提示二者很可能为一种伴存关系.

For many years, whether schistosomiasis of bowel is a precancerous lesion of col-orectal cancer is a controversy problem. This study is to further clarify the relationship between schistosomiasis and colorectal cancer. Methods: Cellular DNA content and products(p21ras, p53 protein) of two oncogenes in chronic schistosomiasis of large bowel and in colorectal carcinomas were investigated using flow cytometry. Results: No obvious abnormality of the cellular DNA content and the oncogene products was discovered in 42 chronic schistosomiasis of large bowel, including 18 those conpanied with ova polyps, and no apparent difference in two aspects above was demonstrated in both 12 colorectal carcinomas associated with schistosomiasis and 11 simple colorectal carcinomas and in the pericancer tissues of them. Conclusions:Our data indicate that chronic schistosomiasis of large bowel may not have the characteristics of malignant transformation which would have as a precancerous condition. It does not suport a direct causulity between schistosomiasis and colorectal carcinomas and suggests that they should largely be co-existent.