长白落叶松8个家系挥发性化合物的比较分析

Comparative Analysis of Volatile Profiles of Eight Families of Larix olgensis

应用SiloniteTM采样罐、动态顶空采集、7100A预浓缩仪、GC-MS系统,研究长白落叶松-1、长白落叶松-2、长白落叶松-3×兴安落叶松-1、长白落叶松-4×兴安落叶松-2、长白落叶松-5×日本落叶松、长白落叶松-6、长白落叶松-7、长白落叶松-8等长白落叶松8个家系挥发性物质的异同。共鉴定出33种挥发性物质,其中萜类化合物17种,烃类化合物16种。各家系挥发性化合物中萜类化合物主要有莰烯、β-月桂烯、1R-α-蒎烯、α-萜品烯、β-水芹烯、γ-萜品烯、(+)-4-蒈烯和大香叶烯D,有的家系还含有三环萜、D-柠檬烯、β-反-罗勒烯、樟脑、乙酸龙脑酯、α-长叶蒎烯、古巴烯、石竹烯、α-石竹烯。不同家系间萜类化合物含量差异较大,由高到低依次为长白落叶松-2,89.65%;长白落叶松-7,84.41%;长白落叶松-4×兴安落叶松-2,78.59%;长白落叶松-5×日本落叶松,68.82%;长白落叶松-6,68.35%;长白落叶松-3×兴安落叶松-1,67.93%;长白落叶松-1,31.34%;长白落叶松-8,24.59%。

In order to compare the component and variation of volatile profiles of different family of Larix olgensis, volatiles from eight families of L. olgensis-1, L. olgensis-2, L. olgensis-3 × L. gmelinii-1, L. olgensis-4 × L. gmelinii-2, L. olgensis-5 × L. kaempferi, L. olgensis-6, L. olgensis-7, L. olgensis-8 were collected with a siloniteTM container by dynamic headspace collection. The collected volatiles were concentrated by using a 7 100A pre-condensable apparatus before they were analyzed and identified with GC-MS. The 33 species of volatiles were isolated and identified with 17 species of terpenoids and 16 species of alkanes. The primary terpenoids in the volatile profiles in the all families were camphene, β-myrcene, 1R-α-pinene, α-terpinen, β- phellandrene, γ-terpinen, （ ＋ ）-4-carene, germacren D. Some families had tricyclene, D-limonene, β-trans-ocimene, camphor, bornyl acetate, α-longipinene, copaene, caryophyllene, α-caryophyllene. The relative percentage and species of terpenoids were obvious difference in the various families. L. olgensis-2 had the highest relative percentage of terpenoids at 89.65% of the collected volatiles with 8 species of terpenoids（ including 5 species of sesquiterpenes）, followed by L. olgensis-7 at 84.41% with 7 species; L. olgensis-4 at 75.59% with 7 species（including 1 species of sesquiterpenes） ; L. olgensis-6, L. olgensis-3 × L. gmelinii-1 and L. olgensis-5 × L. kaempferi at 68.35% with 4 species,67.93% with 5 species（including 2 species of sesquiterpenes）,65.72% with 7 species,respectively; L. olgensis-1 at 31.34% with 1 species; and finally L. olgensis-8 at 24.59% with 5 species（including 1 species of sesquiterpenes）.