摘要
(GFI1 ) 生长因素独立 1 为成年造血的干细胞(HSC ) 的哺乳动物的淋巴细胞和 neutrophils 和维护的成熟是重要的。在胚胎的造血作用的 GFI1 的角色是很好描绘的更少。通过一幅 enhancer 陷井屏幕和生物信息学分析，我们识别了 Gfi1 (命名 gfi1.1 ) 的一个斑马鱼相当或相同的事物并且在胚胎发育期间分析了它的功能。内长的 gfi1.1 基因和在它的 genomic 地点附近插入的 GFP 记者基因的表示在斑马鱼胚胎的造血的房间被检测。Morpholino (瞬间) gfi1.1 击倒 scl， lmo2， c-myb， mpo， rag1， gata1 和血红素高山的减少的表示哈 embryonic-1 (hbae1 ) ，以及胚胎的血红素的全部的数量，而是 pu.1 和 l-plastin 的增加的表示。在一样的条件下面，瞬间注射没影响涉及的标记脉管并且专业版肾的开发。相反地，在经由信使 rna 的 gfi1.1 的表示上，注射提高了 gata1 的表示，但是禁止了 pu.1 的表示。这些调查结果建议 Gfi1.1 在调整胚胎的 erythroid 和 myeloid 系决心的平衡起一个关键作用，并且也在斑马鱼期间为淋巴细胞和 granulocytes 的区别被要求胚胎开始。
Growth factor independence 1 （GFI1） is important for maturation of mammalian lymphocytes and neutrophils and maintenance of adult hematopoietic stem cells （HSCs）. The role of GFI1 in embryonic hematopoiesis is less well characterized. Through an enhancer trap screen and bioinformatics analysis, we identified a zebrafish homolog of Gill （named grill） and analyzed its function during embryonic development. Expression of both an endogenous griLl gene and a GFP reporter gene inserted near its genomic locus was detected in hematopoietic cells of zebrafish embryos. Morpholino （MO） knockdown of gill.1 reduced expression of scl, Imo2, c-myb, mpo, ragl, gatal and hemoglobin alpha embryonic-1 （hbael）, as well as the total amount of embryonic hemoglobin, but increased expression ofpu.1 and l-plastin. Under the same conditions, MO injection did not affect the markers involved in vascular and pronephric development. Conversely, overexpression of gill.1 via mRNA injection enhanced expression ofgatal but inhibited expression ofpu.1. These findings suggest that Gill.1 plays a critical role in regulating the balance of embryonic erythroid and myeloid lineage determination, and is also required for the differentiation of lymphocytes and granulocytes during zebrafish embryogenesis.
