摘要
目的研究结肠癌组织与对应正常结肠组织微小RNA（miRNA）的表达特征及其在结肠癌诊断中的意义。方法取天津市肿瘤医院2004至2007年手术切除的15例晚期结肠癌组织标本及其癌旁正常组织标本。通过定量PCR（qRT-PCR）法检测6例结肠癌及其癌旁正常结肠组织的手术标本中200种miRNA的表达情况，应用问题分析（PAM）法对其他9例结肠癌组织及其癌旁正常结肠组织进行定性判断（癌和非癌）。结果6例结肠癌及其癌旁正常结肠组织中存在132种miRNA，其中47种miRNA在结肠癌发生过程中表达量显著下降（〈0．66）。应用PAM法对9对结肠癌组织／远端正常结肠组织进行定性判断的结果与病理切片诊断结果基本一致（吻合度准确率11／12）。结论miRNA与结肠癌的发生发展有密切的联系，有可能成为一个有效的肿瘤标志物以进行结肠癌的早期预测和诊断。
Objective To investigate the features of expression of miRNAs in colon cancer and corresponding normal tissues and the significance thereof in the diagnosis of colon cancer. Methods Fifteen pair samples of colon cancer and matched adjacent normal tissue were collected during operation. A high-throughput real-time quantitative RT-PCR method designed to detect mature miRNA sequences was adapted to screen miRNA expression patterns in 6 pairs of matched colon cancer and normal adjacent tissue samples. Northern blotting was conducted to verify the accuracy of the real-time qRT-PCR assay. Moreover, a thermostable reverse transcriptase based real-time qRT-PCR assay designed to quantify the miRNA precursors was performed. Problem analysis method was used to quantitatively determine the characteristics （cancer or non-cancer） of the other 9 pair samples. Results Among the more than 200 miRNAs exam-ined, 132 were found to be expressed in both the colon cancer and normal adjacent tissue samples. Compared with normal tissue, 47 kinds of miRNA in cancer were down-regnlated （less than 0.66 ）. The results of qualitative determination on the 9 samples of cancer tissue/normal tissue were con-sistent with the results of pathological examination. Conclusion Closely linked with the pathogenesis and development of colon cancer, miRNA may be a promising diagnostic biomarker in diagnosis of colon cancer.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2008年第24期1683-1686,共4页
National Medical Journal of China
基金
国家自然科学基金资助项目（30772484）
