高脂饮食所致动脉粥样硬化家兔肝组织学损伤评估

Evaluation of Liver Histological Lesions of Atherosclerosis Models in New Zealand White Rabbits

目的评估高脂饮食诱导新西兰大白兔动脉粥样硬化模型的肝组织学损伤。方法14只雄性新西兰大白兔随机分为对照组(n=6)和高脂组(n=8)。对照组基础饲料喂养,高脂组喂饲高脂饲料诱导动脉粥样硬化模型。实验10周末处死所有动物,测定血清转氨酶、血脂和空腹血糖含量,对升主动脉及肝脏进行肉眼及光镜组织学检查,并测量升主动脉内膜/中膜厚度比值。结果与对照组相比,高脂组血清转氨酶、血脂及空腹血糖水平均显著升高(P<0.05);病理学检查发现,高脂组升主动脉内膜/中膜厚度比值(1.13±0.32)显著高于对照组(0.12±0.04,P<0.05),同时合并肝脂肪病变,且肝脏炎症反应程度明显。结论高脂饮食诱导动脉粥样硬化同时,导致肝组织学损伤严重。

Aim To evaluate liver histological lesions of atherosclerosis animal models induced by high lipid diet in New Zealand White rabbits. Methods Normal male New Zealand White rabbits （n=14） were randomly divided into two groups： control group （ n = 6 ） and atheroscleresis group （ n = 8 ）. Atherescleresis was induced by high lipid diet （ 92% normal rabbit chow with 2% cholesterol and 6% lard）. Rabbits were sacrificed at the end of week 10. Serum aminotransferase, lipid levels and fasting plasma glucose （FPG） levels were examined dynamically and morphology changes in ascending aortas and livers were observed. The intima-to-media （I/M） ratio of ascending aortas was also measured. Results The levels of serum amin-otransferase, lipid and FPG levels in atherosclerosis group were significantly higher than those of control group （ P 〈 0.05 ）. Compared with control group, the livers presented the pathology of hepatic steatosis and steatohepatitis in atheresclerosis group, and ascending aortas showed typical athcroscleresis changes. The mean I/M ratio in ascending aortas of atheroscleresis group （1.13±0.32） washigher than control group （0.12±0.04, P〈0.05）. Conclusion High lipid diet can induce an idesl rabbit model of atherosclerosis, and also induce severity liver histological lesion, and may be a right method to create the rabbit model in researching the relationship between fatty liver and atherosclerosis.