激活蛋白-1在不稳定冠状动脉病变中的作用

The role of activator protein-1 in unstable coronary atherosclerotic changes

目的探讨冠心病患者激活蛋白-1与冠状动脉（冠脉）粥样硬化病变的关系。方法根据冠脉造影将142例患者分为冠心病组和对照组。冠心病组根据临床类型、病变类型和病变程度进一步分组。裂解外周血白细胞，测定磷酸化c—Jun吸光度（A），反映活化AP-1数量。结果磷酸化c-Jun A值冠心病组明显高于对照组（1．43±0．33比0．71±0．13，P〈0．001），急性冠脉综合征组明显高于稳定性心绞痛组（1．56±0．28比1．14±0．25，P〈0．001）。磷酸化c-Jun随冠脉病变类型和程度的加重而增加。结论AP-1表达量增高与冠脉粥样硬化发生显著相关，其可能作为预测冠脉粥样硬化病变及斑块稳定性的一个指标。

Objective To investigate the relation between activator protein-1 （ AP-1 ） and coronary atherosclerotic changes and the potential role of AP-1 in the stabilization of atherosclerotic plaques in patients with coronary heart disease （CHD）. Method 142 patients were included in this study and divided into CHD group（ 107 ） and control group （35） according to coronary angiography （CAG）. The CHD group was further divided into a stable angina pectoris （SAP） group （32） and an acute coronary syndrome （ACS） group （75） according to the clinical manifestations, In addition, the CHD group was divided into A type group, B type group and C type group according to the standard of ACC/AHA coronary change in 1988. Meanwhile, the CHD group was further divided into light stenosis group, moderate stenosis group and severe stenosis group according to the degree of coronary lesion. The lysate of cells was obtained through lysis of the leucocyts from peripheral blood with cell lysis buffer. The amount of Phospho-c-Jun in lysate was measured with enzyme-linked immunosorbent assay （ELISA）. The results were demonstrated with absorbance, which reflects the amount of AP-1. Results The main coronary changes in the SAP group were A type （68. 7% ） and the changes were mainly of light degree （53. 1% ） ; the main coronary changes in the ACS group were B type（ 52. 0%） or C type （37. 3%） and the changes were mainly of heavy degree （66. 7%）. The absorbance of Phospho-c-Jun in CHD group was significantly higher than that in the control subjects （ 1.43 ± 0. 33 vs 0. 71 ±0. 13, P 〈 0. 001 ）. The absorbance of Phospho-c-Jun in the ACS group was significantly higher than that in the SAP group （ 1.56 ±0. 28 vs 1.14 ±0. 25, P 〈0. 001 ）. The absorbance of Phospho- c-Jun increased gradually from A type group to C type group （ 1.18 ±0. 27 vs 1.42 ±0. 26 vs 1.71 ±0. 27, P 〈0. 001 ） and from light stenosis group to severe stenosis group （ 1.09 ±0. 20 vs 1.37 ±0. 26 vs 1.60 ± 0. 29, P 〈 0.001 ）. Conclusion There is a significant relationship between AP-1 and coronary atherosclerotic changes. AP-1 may be a factor that can predict coronary arteriosclerotic progression and stability of the plaque.