羟基脲对大鼠Sertoli-germ细胞共培养的影响

Effect of hydroxyurea on rat Sertoli-germ cell Co-culture

目的:观察羟基脲(Hydroxyurea,HU)对雄性大鼠Sertoli-germ细胞共培养的细胞毒性作用。方法:在Sertoli-germ细胞共培养模型中分别加入不同浓度HU(0、10-3、10-2、10-1mol/L)后,孵育96h;以及加入10-2mol/LHU后分别孵育24、48、72、96h。结果:在各给药组,96h时HU导致germ细胞从Sertoli细胞层的脱落增加,呈现量效关系。在10-3mol/L以上浓度组,细胞活性逐渐下降,细胞脱落增加。同样,随着给药时间的增加,germ细胞脱落率和乳酸脱氢酶(LDH)的漏出逐渐增加,细胞活性逐渐下降。结论:HU对雄性大鼠睾丸Sertoli-germ细胞共培养模型的germ细胞具有明显毒性作用。

AIM： to evaluate the cytotoxic effects of Hydroxyurea （HU） in vitro employing rat testicular Sertoli-germ cell cocuhure. METHODS： Cytotoxio changes induced by HU （0, 10^-3, 10^-2, 10^-1mol/L） in mixed cultures of Sertoli and germ cells were seen at 96 h after treatment, respectively. And 10^-2 mol/L HU were added to the culture, at 24, 48, 72 and 96 h after treatment, respectively. RESULTS： HU led to an increase in germ cell detachment from the Sertoli cell monolayer in a dose dependent manner. Detached cells were observed and the viability of detached cells was decreased in 10^-3 mol/L and above treated groups. Substantial leakage of the cytosolic enzyme lactate dehydrogenase （LDH） was observed in the medium after 96 h HU treatment at concentrations of 10^-2mol/L and above treated groups. The same, HU exposure led to an increase in germ cell detachment, the leakage of LDH, a decrease in cell viability from the Sertoli cell monolayer in a time dependent manner compared with the control culture. CONCLUSION： It is shown that HU exhibited an obvious toxicity to the germ cells of male rats in Sertoli cell germ cell co-culture model.