我国藏族和汉族儿童少年骨转换研究

Biomarkers on bone turnover in Chinese Tibetan and Han nationality children and adolescents

目的探讨我国藏族和汉族8～18岁儿童少年骨形成和骨吸收指标的变化规律。方法采用分层随机整群抽样,随机抽取四川省阿坝藏羌自治州718名8～18岁的藏族和汉族儿童少年,各年龄各民族各性别为20人左右。采用英国IDS公司生产的酶联免疫试剂盒测定血清中骨碱性磷酸酶（BAP）和抗酒石酸盐酸性磷酸酶（TRAP）浓度。结果研究对象平均年龄12.8±3.0岁,血清BAP浓度在男生平均为80.6±40.4μg/L,女生为72.3±39.9μg/L;血清TRAP浓度在男生平均为8.5±4.3 U/L,女生为7.9±4.5 U/L。血清BAP或TRAP浓度受民族、年龄和性别的共同影响,分别解释变异的27.0%或35.7%（P〈0.001）。8岁开始女生BAP或TRAP浓度高于男生,分别在10.0岁或10.5岁出现交叉,之后男生开始高于女生。汉族儿童比藏族的BAP平均低10μg/L,TRAP低2.56～2.96 U/L。结论我国8～18岁藏族和汉族儿童少年骨形成和骨吸收指标都受年龄、性别和民族共同影响,男女生随年龄增长存在交叉,藏族高于汉族。

Objective To observe the biomarkers on bone formation and absorption in Chinese Tibetan and Han nationality children and adolescents.Methods In a cross-sectional study,718 subjects were randomly recruited in A-Ba Zang Qiang Autonomous Prefecture,Sichuan Province,China,with about 20 subjects per nationality,gender and age.The concentration of serum Bone-specific Alkaline Phosphatase（BAP） and Tartrate-resistant Acid Phosphatase（TRAP） were measured by ELISA kits of IDS.Results The average age of subjects were 12.8±3.0 y.The concentration of serum BAP were 80.6±40.4 μg/L for boys and 72.3±39.9 μg/L for girls,on average.The average serum TRAP concentration were 8.5±4.3 U/L for boys and 7.9±4.5 U/L for girls.The concentration BAP or TRAP were associated with age,gender,and nationality（R2=0.270 or 0.357,P〈0.001）.The concentration of BAP or TRAP were higher in girls than in boys,crossed at 10.0 y or 10.5 y,and then higher in boys.The different between Tibetan and Han nationality were 10μg/L for BAP,and 2.56-2.96 U/L for TRAP.Conclusion Gender,sex and nationality have effects on serum concentration of BAP or TRAP in Chinese Tibetan and Han nationality children and adolescents,with higher in Tibetan and a cross with age between boys and girls.