聚乙二醇-牛血红蛋白停搏液对离体心脏功能和超微结构的影响

Effect of Polyethylene Glycol-Bovine Hemoglobin Cardioplegic Solution on Cardiac Function in Vitro

目的观察含聚乙二醇-牛血红蛋白(polyethylene glycol-bovine hemoglobin,PEG-bHb)的心脏停搏液对离体心脏功能的影响。方法32只成年雄性S-D大鼠根据停搏液不同随机分为4组,麻醉后开胸取心,建立Langendorff离体心脏灌注模型,平衡20 min后灌注冷St.Thomas液或三种不同浓度的PEG-bHb停搏液,30℃保持60 min后恢复灌注。记录各组左室发展压(LVDP)、左心室内压最大变化速率(±dp/dtmax)和冠脉流出量(CF)等血流动力学指标,并行超微结构观察。结果恢复灌注后,三个PEG-bHb组的LDVP、±dp/dtmax及CF均显著高于St.Thomas液组(P<0.05),超微结构也得到较好的保护。三个PEG-bHb组之间除CF外,其余指标均无明显差异(P>0.05)。结论以PEG-bHb制成的含血停搏液可以改善离体心脏的血流动力学表现和超微结构。

OBJECTIVE To evaluate the effect of polyethylene glycol-bovine hemoglobin（PEG-bHb）-containing cardioplegic solution on cardiac function in isolated animal hearts.METHODS The hearts of 32 male S-D rats were harvested and transferred to langendorff circuit.They were divided into 4 groups according to cardioplegia： St.Thomas or 3 different concentrations of PEG-bHb in St.Thomas solution.After 20 min balance period,hearts were perfused with cold（4℃） cardioplegic solutions,and preserved at 30℃ for 60 min,then reperfused.RESULTS After reperfusion,left ventricular developed pressure（LVDP）,derivative of pressure（±dp/dtmax） and coronary flow（CF） were higher in PEG-bHb groups（P〈0.05）.Ultrastructures were better preserved in PEG-bHb groups.There was no difference among 3 PEG-bHb groups except CF.CONCLUSION PEG-bHb in cardioplegic solutions can improve hemodynamic values and ultrastrctures in hearts after ischemia.