高铁血红素对体温过低症犬的保护作用研究

Protective Effect of Hemin on Hypothermia Dogs

目的探讨高铁血红素对海水浸泡性体温过低症犬的保护作用。方法健康杂种犬12只,随机分为对照组和高铁血红素组。高铁红素组手术前48 h腹腔注射高铁血红素(每次80μmol/kg,2次/天,2天)。采用海水浸泡性体温过低症模型,将肛温降至30℃,于实验前和实验后肛温30℃时分别取血浆测定超氧化物歧化酶(SOD)、总胆红素(TB)、谷草转氨酶(GOT)、乳酸脱氢酶(LDH)、肌酸激酶(CK)、肌酸激酶同功酶(CKMB)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的含量。结果高铁血红素组腹腔注射高铁血红素2天后,制作低温模型前在肛温39℃时,与对照组比较,SOD明显升高,组间差异有统计学意义(P<0.05)。制作体温过低症模型将两组动物肛温降至30℃时,高铁血红素组SOD、TB高于对照组(P<0.05);GOT、LDH、CK、CKMB、IL-6、TNF-a低于对照组(P<0.05)。结论血红素氧合酶的诱导剂高铁血红素可在一定程度上提高血胆红素水平,增加SOD活性,提高血清总抗氧化能力,对低温损伤有一定的保护作用。

OBJECTIVE To explore the protective effect of hemin on hypothermic dogs immersed by seawater.METHODS Twelve healthy dogs were devided into two groups： control group and hemin group.To hemin group,hemin（80 μmol/kg,twice a day）were administered through intraperitoneal injection for 2 days before hypothermia model was made.No hemin was used to control group.Then immersed hyperthermia model was made in two groups.Dogs were immersed in cold seawater（12 ±2）℃ until the rectal temperature was decreased to 30℃.Plasma total bilirubin（TB）,superoxide dismutase（SOD）,aspartate aminotransferase（GOT）,lactate dehydrogenase（LDH）,creatine kinase（CK）,CKMB,interleukin-6（IL-6） and tumor necrosis factor（TNF-α）contents were measured before and after the model was made.All the data were compared between two groups.RESULTS The content of TB and SOD of hemin group was higher than that of control group at 30℃（P〈0.05）.The content of GOT,LDH,CK,CKMB,IL-6 and TNF-α of hemin group was lower than that of control group at 30℃（P〈0.05）.CONCLUSION Hemin could exert protective effect on seawater immersed hypothermic dogs through elevating the serum level of SOD.