VP16对S-180和S-180-R细胞凋亡的作用

EFFECT OF VP16 ON APOPTOSIS OF S 180 AND S 180 R CELLS

①目的探讨ＶＰ１６对细胞凋亡的作用。②方法用两种剂量（３．１２，６．２５μｍｏｌ）的ＶＰ１６处理腹腔接种Ｓ－１８０瘤细胞和抗阿霉素瘤细胞（Ｓ－１８０－Ｒ）的ＢＡＢＬ／Ｃ鼠。０，６，９ｈ分别取腹水，用流式细胞仪测定细胞相对ＤＮＡ含量。③结果ＶＰ１６在３．１２，６．２５μｍｏｌ剂量时，均可诱导Ｓ－１８０细胞凋亡，而剂量效应并不明显。在６．２５μｍｏｌ时，也可诱导Ｓ－１８０－Ｒ细胞凋亡，且ＶＰ１６诱导Ｓ－１８０和Ｓ－１８０－Ｒ两种细胞凋亡差别不明显。④结论ＶＰ１６是Ｓ－１８０和Ｓ－１８０－Ｒ细胞凋亡的诱导剂。ＶＰ１６诱导的凋亡与其抗药表型关系不大，但与免疫机制似乎有一定的关系。

Objective To study the effect of VP16 on apoptosis. Methods BABL/C mice previously inoculated with parental S 180 and adriamycin resistant S 180 R cells were treated with two different dosages of VP16. S 180 and S 180 R cells drawn from the abdominal cavity at 0, 6, 9h and analyzed by flow cytometer FACS 420 for DNA content. Results VP16 could induce apoptosis in S 180 cells and the inducing effect of both dosages showed no significant difference. For S 180 R cells, only VP16 at dosage of 6.25μmol could induce apoptosis. The inducing effect of VP16 for both S 180 and S 180 R cells showed no significant difference. There was an increase of lymphocyte in the abdominal cavity along with the appearance of cell apoptosis. Conclusion VP16 is an apoptosis inducer for S 180 and S 180 R cells. Under present experiment condition, the apoptosis induced by VP16 is not closely correlated with the drug resistance phenotype of cells, but has certain relationship with the immune system.