卵巢癌流式细胞DNA倍体和S期比例的异质性

FLOW CYTOMETRIC DNA PLOIDY AND S PHASE HETEROGENEITY IN OVARIAN CANCER

①目的探讨卵巢癌流式细胞ＤＮＡ倍体和Ｓ期比例的异质性。②方法应用流式细胞术（ＦＣＭ）测定了２７例卵巢癌的ＤＮＡ倍体和Ｓ期比例（ＳＰＦ），每例测定２～６块，取自原发癌和腹腔转移癌的不同区域石蜡包埋肿瘤组织。③结果２１例ＤＮＡ指数一致，６例显示ＤＮＡ指数异质性；ＤＮＡ异倍体肿瘤中只有９例可进行ＳＰＦ计算，结果同一病人不同区域ＳＰＦ变异很大，其中位数为８％（２％～１５％）；所有ＤＮＡ二倍体肿瘤的ＳＰＦ变异中位数为３％（０～７％）。④结论为保证流式细胞ＤＮＡ倍体及ＳＰＦ测定能代表卵巢癌的全部细胞干系，应采取多区域取材检测。

Objective To study the flow cytometric DNA ploidy and S phase heterogeneity in ovarian cancer. Methods DNA ploidy and S phase fraction(SPF) from 27 patients with epithelial ovarian carcinoma were analyzed by flow cytometry(FCM).Two to six paraffin embedded tissue blocks from each primary tumor and its metastatic foci were obtained. Results Stable DNA index (DI) was found in 21 patients, heterogeneity was found in 6 patients. SPF measurements were possible only in 9 cases of DNA aneuploid and whose SPF value varied considerably among different samples from the same patient. The median SPF value was 8% (2%～15%). The median SPF value in all DNA diploid cases was 3% (0%～7%) . Conclusion To ensure that all tumor stem lines are represented, multiple biopsy specimens from the primary tumor and its metastatic foci should be analyzed by flow cytometry and SPF measurement.