摘要
目的:观察一次性运动对胰岛素抵抗大鼠骨骼肌磷脂酰肌醇3-激酶mRMA表达、蛋白总量及磷酸化PI3K的影响。方法:SD大鼠随机分为正常饮食组、高脂饮食组、高脂饮食+运动组。分别对高脂饮食组与高脂饮食+运动组饲以高脂饲料4周后,对高脂饮食+运动组大鼠运动干预2h。测血液葡萄糖和胰岛素浓度;Western blot法检测骨骼肌t-PI3K蛋白表达p、-PI3K磷酸化水平;RT-PCR法分析PI3K mRNA表达。结果:与正常对照组比较,高脂饮食组胰岛素浓度升高;高脂饮食+运动组胰岛素浓度低于高脂饮食组;高脂饮食组PI3KmRNA表达低于正常对照组;高脂饮食+运动组PI3K磷酸化水平高于高脂饮食组。结论:运动干预改善高脂饮食诱发胰岛素抵抗大鼠对胰岛素的敏感性,增加PI3K磷酸化水平。
Objective: To investigate the effects of a single bout of exercise on PI3K phosphorylation, protein and gene expres- sion in skeletal muscle of insulin-resistance rats. Methods: Male rats were randomly divided into control and high-fat diet and high-fat diet trained groups. The rats were fed a high-fat diet (HFD) for 4 weeks and then the rats in exercise group exer- cised for 2 h swimming, The serum levels of glucose and insulin were measured. The levels of PI3K protein and phosphory- lation were determined by Western blot, and the level of PI3K mRNA was determined by reverse transcription polymerase chain reaction. Results: Compared with control group, the serum levels of insulin in HFD rats was increased. The serum lev- els of insulin of HFD rats with exercise training was significantly lower than that in HFD rats. The level of PI3K mRNA in HFD rats was decreased as compared to control. The levels of PI3K phosphorylation in HFD rats with exercise training were higher than that in HFD rats. Conclusion:Exercise may improve PI3K phosphorylation and increase the sensitivity of skele- tal muscle to insulin.
出处
《沈阳体育学院学报》
2008年第3期44-45,56,共3页
Journal of Shenyang Sport University
基金
辽宁省教育厅高等学校科研基金资助项目(2005L423)
关键词
胰岛素抵抗
运动
磷脂酰肌醇3-激酶
骨骼肌
insulin-resistance
exercise
phosphatidylinositol 3 kinase
skeletal muscle