氯化镉对大鼠肾细胞内MAPK基因mRNA表达的影响

Effects of cadmium chloride on the expression of MAPK mRNA in NRK cells

目的观察不同浓度氯化镉(CdCl2)对大鼠肾(NRK)细胞MAPK基因mRNA表达的影响。方法应用四甲基偶氮唑盐(MTT)比色法检测不同浓度CdCl2(0、2.5、5.0、10.0、20.0和40.0μmol/L)染毒24h后对NRK细胞存活率的影响;应用带有SYBRGreenⅠ的实时荧光定量PCR技术观察不同浓度CdCl2(0、2.5、5.0和10.0μmol/L)对NRK细胞内MAPK基因(ERK1/2、p38MAPK、JNK1/2)mRNA表达的影响。结果以阴性对照组(CdCl20μmol/L)NRK细胞存活率为100%,各染毒组(CdCl25.0、10.0、20.0和40.0μmol/L)NRK细胞存活率分别为72.28%、39.95%、15.66%、10.39%,且与对照组之间差异有统计学意义(P<0.01),并存在剂量—效应关系;经CdCl2染毒后,各实验组(CdCl22.5、5.0和10.0μmol/L)NRK细胞的ERK1/2、p38MAPK、JNK1/2基因的mRNA的相对表达量均低于阴性对照组(P<0.05或P<0.01)。结论CdCl2可能引起NRK细胞内MAPK基因mRNA的表达水平发生改变。

Objective To observe the effect of cadmium chloride （ CdCl2 ） on the expression of MAPK mRNA in rat NRK cells. Methods MTT assay was conducted to detect the effects of CdCl2 at various concentrations on the survival rate of NRK cells after 24 hours. Real - time fluorescence quantitative PCR with SYBR Green I technology was introduced to measure the effects of CdC12 on the expression of MAPK（ERK1/2, p38MAPK, JNK1/2 ）mRNA in NRK cells exposed to CdCl2 at various concentrations. All the results of different concentration were compared. Results The survival rate of NRK cells were 72.28%, 39.95%, 15.66%, 10.39%, respectively in different concentrations of CdC12 （5.0,10. 0, 20. 0,40. 0 umoL/L） after 24 hours, while 100% in the negative control group. There existed significant difference and dose response relationship between treated and control groups （ P 〈 0. 01 ）. The mRNA expression of MAPK （ERK1/2, p38MAPK, JNK1/2） was lower than the negative control group after treated with CdCl2 （ P 〈 0.05, P 〈 0.01 ）. Conclusion CdCl2 could change expression of MAPK mRNA in NRK cells.