恶性肿瘤胸腹水中TIL的体外扩增及抗肿瘤作用

IN VITRO PROLIFERATION OF TUMOR INFILTRATING LYMPHOCYTES FROM HUMAN MALIGNANT PLEURAL EFFUSION AND ASCITES AND STUDY OF THEIR ANTI TUMOR EFFECT

①目的探讨恶性肿瘤胸腹水中肿瘤浸润淋巴细胞（ＴＩＬ）的体外诱导扩增及其抗肿瘤作用。②方法用不连续密度梯度离心法从９例癌性胸水和６例癌性腹水中提取ＴＩＬ进行体外诱导培养，同时用ＭＴＴ单波长比色法测定ＴＩＬ的活性。③结果ＴＩＬ对自体肿瘤靶细胞的杀伤活性达７６％以上，且呈现一定的靶细胞特异性，体外扩增可达１１００倍。④结论ＴＩＬ对肿瘤细胞有明显的杀伤活性，其活性高峰期在诱导培养的７～１５ｄ．

Objective To investigate the in vitro proliferation method of tumor infiltrating lmphocytes( TIL) from malignant pleural effusion and ascites and study of their anti tumor activity. Methods Density gradient centrifugation was used to separate TIL from 9 samples of malignant pleural fluid and 6 samples of malignant ascitic fluid.TIL were cultured in vitro and changes in cytotoxic activity were determined by MTT assay. Results The cytotoxic activity of TIL against autologous tumor cells was more than 76% and exhibited specificity for target cells. In vitro proliferation could reach 1100 times. Conclusion TIL exhibited specific cytotoxic activity against tumor cells and the anti tumor activity was at the highest level between 7￣15 days.