急性心肌梗塞病人血清CK-MM亚型的检测

DETECTION OF SERUM CK MM ISOFORMS IN PATIENTS WITH ACUTE MYOCARDIAL INFARCTION

①目的观察急性心肌梗塞（ＡＭＩ）病人血清肌酸磷酸激酶（ＣＫ）及其亚型的变化，以探讨对ＡＭＩ早期诊断及病情变化的意义。②方法应用琼脂糖凝胶电泳法检测２０例ＡＭＩ病人血清ＣＫ总活力及其亚型ＭＭ变化，并与对照组和心绞痛组进行了比较。③结果ＡＭＩ发病３ｈ内，ＣＫ总活力和ＣＫ－ＭＢ与对照组和心绞痛组均无明显改变，而ＭＭ３及ＭＭ３／ＭＭ１比值则明显高于上述两组（ｔ＝７．８１，１０．０８，Ｐ均＜０．０１）。ＡＭＩ发病后，ＣＫ总活力和ＣＫ－ＭＢ达到峰值时间分别为２３．２和２１．６ｈ，而ＭＭ３／ＭＭ１则在１２．３ｈ达到峰值，ＭＭ３／ＭＭ１比值达峰值时间明显缩短（ｔ＝７．４５，Ｐ＜０．０１）。④结论检测ＣＫ及其亚型的变化有助于对ＡＭＩ的早期诊断和病情判断。

Objective To observe the changes of serum creatine kinase isoforms after the onset of acute myocardial infartion(AMI) . Methods Changes of serum total CK and CK isoforms from 20 patients with acute myocardial infarction were detected with agrose gel electrophoresis method and compared with normal subjects and patients with angina pectoris respectively. Results Within 3 hours after heart attack, the serum total CK and CK Mb showed no significant difference compared with that of AMI patients, normal subjects and angina pectoris patients. But MM 3 and MM 3/MM 1 were significantly higher than that of the two control groups( t=7.81, 10.08; P <0.01). Total CK and CK Mb of AMI patients reached the peak value at 23.2 and 21.6 h, while MM 3/MM 1 reached the peak value at 12.3h. The time difference was significant( t=7.45, P<0.01). Conclusion Detection of CK MM isoforms is helpful for diagnosis of AMI and for assessing the conditions of patients.