乙型肝炎表面抗原阳性血清经不同方法消毒后用PCR法对HBV-DNA检测结果

RESULTS OF EXAMINATION OF HBV-DNA IN HBsAg-POSITIVE SERUM BY PCR METHOD AFTER DISINFECTION WITH VARIOUS METHODS

经用ＰＣＲ法技术检测，以辐照度值＞１０５μＷ／ｃｍ２的紫外线对ＨＢｓＡｇ阳性血清照射８小时，对ＨＢＶ－ＤＮＡ有一定灭活作用。煮沸１０分钟，或者以７５％乙醇作用１小时，对ＨＢＶ－ＤＮＡ灭活能力较差。

Examination by PCR technique indicated that exposure of HBsAg-positive serum to ultraviolet rays with a radiation intensity of >105 μW/cm 2 for 8 hr had certain inactivating effect on HBV-DNA. Boiling for 10 min or exposure to 75% ethanol for 1 hr was more difficult to inactivate HBV-DNA.