摘要
目的研究壳聚糖及其衍生物壳寡糖、羧甲基壳聚糖(CM-chitosan)、N-乙酰氨基葡糖、氨基葡糖盐酸盐体外清除羟自由基的能力。方法采用邻二氮菲-Fe2+氧化法。结果在实验设置的浓度范围内,清除羟自由基的能力依次为壳寡糖>壳聚糖>N-乙酰氨基葡糖>氨基葡糖盐酸盐>羧甲基壳聚糖,清除能力均随浓度增加而增大。以0.32 mg/mL壳寡糖的清除率最大,达到97.81%,相当于0.64 mmol/L抗坏血酸清除羟自由基的能力。结论本实验为开发壳聚糖及其衍生物作为抗氧化能力较强的食品和药品奠定了一定基础。
Objective The antioxidative activities in vitro of chitosan, caboxymethyl-chitosan (CM-chitosan), oligo-chitosan, N-acetyl-glucosamine (NAG) and glucosamine hydrochloride (GlcNH2 · HCl) were investigated. Methods Phenanthroline-Fe2+ method was used. Results The results showed that in the setting concentration range, the antioxidative activities increased with the elevation of concentration. The activity of scavenging hydroxyl free radical of chitosan and its derivatives was that oligo-chitosan 〉 chitosan 〉 NAG 〉 GlcNH2 ~ HC1 〉 CM- chitosan and the highest rate of hydroxyl free radical-scavenging of 0.32 mg/mL oligo-chitosan could be 97.81%, which was similar with the activity of 0.64 mmol/L vitamin C. Conclusion Our work provided the theory basis for further application of chitosan and its derivatives on food and drug.
出处
《食品与药品》
CAS
2008年第4期23-24,共2页
Food and Drug
基金
辽宁省教育厅高校科研计划资助项目(20060153)
大连民族学院博士科研启动基金资助项目(20056106)
大连民族学院"太阳鸟"项目
关键词
壳聚糖
衍生物
抗氧化性
羟自由基
chitosan
derivatives
antioxidative activity
hydroxyl free radical