摘要
目的测定人参源胶囊中人参皂苷Rh2的含量。方法采用反相高效液相色谱法,色谱柱:ODS-C18(5μm,250 mm×4.6 mm),流动相:乙腈-水(60:40),流速:1.0 mL/min,检测波长:203 nm,柱温:30℃。结果人参皂苷Rh2在0.08～0.24 mg/mL的浓度范围内,其进样量与峰面积呈良好的线性关系,r=0.999 2,平均回收率为9 9.7 5%(n=6)。结论本法简便、快捷、准确,重复性好,可用于人参源胶囊含量的测定和质量控制。
Objective To determine the content of ginsenoside Rh2 in Ginseng Capsules. Methods A RP-HPLC method was adopted with ODS-C18 column （5 μm, 250 mm × 4.6 mm）, mobile phase of acetonitrile-water （60： 40）, flow rate of 1.0 mL/min and column temperature of 230oc. The detection wavelength was 203nm. Results There was a good linear relationship for ginsenoside Rh2 in the range of 0.08 mg/mL - 0.24 mg/mL（r = 0.999 2） and the average recovery was 99.75% （n=6）. Conclusion This method is simple, rapid and accurate for the assaying and quality control of Ginseng Capsules with a good reproducibility.
出处
《食品与药品》
CAS
2008年第4期35-37,共3页
Food and Drug
