炎症性肠病患者淋巴毒素α、白介素1受体拮抗剂基因多态性与肿瘤坏死因子α,可溶性白介素2受体以及白介素6产量的关系

Relation of Polymorphisms of Lymphotoxin α and Interleukin 1 Receptor Antagonist Genes to Secretion of Tumour Necrosis Factor α, Soluble Interleukin 2 Receptor and Interleukin 6 in Chinese Patients with Inflammatory Bowel Disease

测定中国炎症性肠病患者淋巴毒素α基因和白介素１受体拮抗剂基因多态性与肿瘤坏死因子α、可溶性白介素２受体以及白介素６产量的关系。２２例炎症性肠病患者（２０例溃疡性结肠炎，２例克隆病）和１０例健康对照者参加研究。淋巴毒素α基因和白介素１受体拮抗剂基因片段由基因组ＤＮＡＰＣＲ扩增而来，采用ＥＬＩＳＡ法测定周围血单个核细胞肿瘤坏死因子α，可溶性白介素２受体以及白介素６产量。结果：炎症性肠病淋巴毒素α基因型１和２杂合子略高于正常对照组（１１／１２对１／１０，Ｐ＝００４９）；而且淋巴毒素α等位基因２与周围血单个核细胞诱生的高水平肿瘤坏死因子α产量有关。白介素１受体拮抗剂基因多态与炎症性肠病无显著性相关。认为：中国人群炎症性肠病患者淋巴毒素α基因可能对肿瘤坏死因子α产量起一定作用。

? To determine the relation of lymphotoxin α and interleukin 1 receptor antagonist genes to the secretion of tumour necrosis factor α, soluble interleukin 2 receptor and interleukin 6 in Chinese patients with ulcerative colitis. Patients and Methods: Twenty_two patients with inflammatory bowel disease(20 ulcerative colitis and 2 Crohn's disease) and 10 healthy ontrols were studied. Lymphotoxin α gene and interleukin_1 receptor antagonist gene fragments were amplified from genomic DNA by PCR. Tumour necrosis factor α, soluble interleukin 2 receptor and interleukin 6 production from peripheral blood mononuclear cells were measured by ELISA’ s. Results: The genotype 1 and 2 of lymphotoxin α was slightly higher in inflammatory bowel disease than in the healthy control (11/22 vs 1/10, P=0.049) and allele 2 of lymphotoxin α was related to higher tumour necrosis factor α production from peripheral blood mononuclear cells on stimulation. There was no association between inflammatory bowel disease and interleukin 1 receptor antagonist gene polymorphism. conclusion: Lymphotoxin α gene may play a role in relation to secretion of tumour necrosis factor α in Chinese patients with inflammatory bowel disease.