摘要
采用纯化的腺病毒抗原包被固相载体以辣根过氧化物酶标记的抗人IgA结合物作为第二抗体,建立了腺病毒IgAELISA检测法,对41例患儿血清进行了检测,腺病毒IgA阳性率为390%,正常儿童为32%,指出腺病毒仍是婴幼儿呼吸道感染的重要原因之一。
The detection method for adenovirus specific IgA antibody was established with purified adenovirus antigen to coat solid microplate and anti_human IgA as second antibody. The results showed that the positive rate of adenovirus IgA is 39.0% in infants infacted respiratory virus, but the rate is 3.2% in healthy children. This indicates that the adenovirus is a main pathogen in respiratory infection in children. This method is specific sensitive, simple and realiable.