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鸡肌抑素基因的原核表达及蛋白纯化 被引量：5

Expression and purification of myostatin gene in chicken
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摘要 利用实验室保存的重组质粒pMD18-MSTN重新构建原核表达质粒pET-32a-MSTN,将其转化到宿主菌大肠杆菌BL21上并进行诱导表达,再将所表达的融合蛋白进行纯化。结果表明:该基因在宿主菌中成功表达;目的蛋白被成功纯化,且纯度较高。 The recombinant plasmid pET- 32a- MSTN was eonstructed aeeording to the plasmid pMD18 -MSTN preserved in our laboratory and then was transformed into BL21 to express target protein. The result showed that the gene ean be expressed in E. eoli. The expressed produet was purified. Therefor the target protein can be purified and the purity was on a high level.
作者 蓝赐华 刘为民 梁梓森
机构地区 佛山科学技术学院动物医学系 华南农业大学兽医学院
出处 《黑龙江畜牧兽医》 CAS 北大核心 2008年第7期18-20,共3页 Heilongjiang Animal Science And veterinary Medicine
基金 国家自然科学基金项目(30571341)
关键词 南海黄鸡 肌抑素基因 原核表达 蛋白纯化 Nanhai yellow ehieken myostatin prokaryotie expression protein purifieation
分类号 S831 [农业科学—畜牧学]
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参考文献4

  • 1McPHERRON A C, LEE S J. Double muscling in cattle due to mutations in the myostatin gene [ J ]. Proc Natl Acad Sci, 1997,94 : 12457 - 12461.
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同被引文献59

  • 1吕文发,袁天祥,孔振兴.牛肌肉生成抑制素基因功能区的克隆与原核表达[J].中国兽医科学,2006,36(6):482-484. 被引量：3
  • 2McPherron AC, Lawler AM, Lee SJ. Regulation of skeletal muscle mass in mice by a new TGF-β superfamily member. Nature, 1997, 387:83-89.
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  • 10Joulia D, Bernardi H, Garandel V, et al. Mechanisms involved in the inhibition of myoblast proliferation and differentiation by myostatin. Exp Cell Res ,2003,286 ( 2 ) :263-275.

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黑龙江畜牧兽医
2008年 第7期

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