虎刺梅的组织培养及快速繁殖初报

Preliminarily Study on the Tissue Culture and Rapid Propagation of Euphorbia milii var.Splendena

[目的]研究虎刺梅的组织培养和快速繁殖。[方法]以虎刺梅子房为外植体,在培养基中添加不同浓度的6-BA、NAAI、BA进行愈伤组织的诱导和快速繁殖。[结果]1/2MS培养基附加6-BA 2.0 mg/L对子房愈伤组织诱导效果最佳;将产生的愈伤组织块转入分化培养基MS＋6-BA 1.0 mg/L＋NAA 0.3 mg/L中,培养45-50 d后,形成了10多个侧芽;在MS＋6-BA 2.0 mg/L＋NAA 0.3 mg/L进行小苗的快速增殖;将芽转接到生根培养基MS＋IBA 0.8 mg/L中,20 d后,产生5-6条根;驯化及移栽所用基质为草炭∶砂子∶珍珠岩=3∶1∶1,7 d后小苗产生了新根,进一步管理可发育成大苗。[结论]该研究利用组织培养实现了虎刺梅的快速繁殖。

[Objective] The research aimed to study the tissue culture and rapid propagation of Euphorbia milii var.Splendena.[Method] With the ovary of E.milii as explant,6-BA,NAA and IBA at different concn.were added to the medium to make the callus induction of rapid propagation.[Result] The effect of appending 2.0 mg/L 6-BA to 1/2 MS medium was best for inducting the ovary callus.The produced callus blocks were transferred into the differentiation medium MS＋1.0 mg/L 6-BA ＋0.3 mg/L NAA and more than 10 lateral buds were formed after 45～50 d.The rapid propagation of seedlings were made on the medium of MS ＋2.0 mg/L 6-BA ＋0.3 mg/L NAA,and then,the buds were transferred into the rooting medium of MS＋0.8 mg/L IBA and 5～6 roots were produced after 20 d.The used substrate for the domestication and transplanting was grass charcoal：sand： perlite of 3∶1∶1 and new roots were produced after 7 d and could be developed into big seedlings through further management.[Conclusion] This research realized the rapid propagation of E.milii through the tissue culture.