低温胁迫对甘薯s-腺苷甲硫氨酸合成酶mRNA表达水平的影响

Effect of Low Temperature Stress on Sweet Potato S-Adenosyl Methionine Synthetase Gene Expression

[目的]为研究s-腺苷甲硫氨酸合成酶基因在冷胁迫条件下的表达情况。[方法]分别提取4℃胁迫处理0、12、24、48和72 h后的甘薯叶、茎和块根组织总RNA,通过反转录和实时定量PCR技术分析了该基因的mRNA表达情况。[结果]叶、茎和块根组织中该基因表达量都明显升高,分别于胁迫后24、72和72 h达到表达高峰,其中块根组织表达量增加量最大。[结论]低温胁迫能够促进相关基因的mRNA表达水平升高。

[ Objective ] The mRNA expression level changes of S-adenosylmethionine synthetase （SAMS） trader low temperature stress was studied. [ Method ]Total RNA were extracted from leaves, stem and earthnut of sweet potato 0,12,24,48 and 72 h after low temperature treatement, mP,.NA expression level was analyzed by reverse expression and Real-time PCR technique. [Result]The expression quality of the gene extracted from leaves, stem and earthnut increased and the expression quality reached the peak point 24,72 and 72 h after low temperature treatment respectively.The expression change of earthnut was the biggest. [Conclusion]Low temperature was good for increasing mRNA expression of relevart genes.