枸杞nrDNA ITS测序鉴定的初步研究

Preliminary Studies on Identification of Lycium Linn. Germplasm Resources by nrDNA ITS Sequencing

[目的]对枸杞nrDNA ITS(核糖体DNA基因内转录间隔区)序列进行分析,从而在分子水平对枸杞做出鉴定。[方法]采用改良CTAB法提取枸杞叶片DNA,利用合成的特异引物对其DNA中nrDNA ITS区进行扩增、克隆,对目的片段测序分析。[结果]克隆到枸杞nrDNA ITS片段并获得其碱基序列,成功找到3个供试枸杞种质材料的nrDNA ITS序列差异。[结论]枸杞nrDNAITS区的扩增和测序可以在分子水平对枸杞不同种质进行鉴别。

[Objective] The study aimed to identify wolfberry （Lycium Linn. ） germplasm resources at molecular level by analyzing the nrDNA ITS sequence. [ Method] Genomic DNAs from wolfberry leaves extracted by modified CrAB method were regarded as templates for PCR amplification by specific primer, clone and sequencing. [Result] The arDNA ITS sequences were obtained and then differentiated among three tested materials. [Conclusion] PCR amplification and sequencing on nrDNA ITS is a feasible approach to identify different woltberry germplasm resources.